The enzyme-linked immunospot (elispot) assay is a very sensitive immunoassay which measures the frequency of cytokine-secreting cells at the single-cell level.
Elispot assays are also independent of secretion kinetics, a significant fact given the unsynchronized nature of the responding t cells pool for ics, all cells are killed via fixation at a pre-determined time.
Principles of the assay the cytokine elispot is both a quantitative and qualitative assay, it is based on the enzyme-linked immunosorbent technique and is designed to enumerate cytokine-secreting cells it is extremely sensitive and therefore useful in detecting low frequency cytokine-secreting cells (1/300 000. Immunospot® applications elispot assays the elispot assay is the most sensitive technique available for the detection of immune cells which secrete specific signature proteins such as antibodies or cytokines.
Currently, elispot assays are performed for applications ranging from basic research to clinical diagnostics as a highly sensitive and effective method for detection of low-frequency cell subsets on a single-cell level, including t and b lymphocytes, monocytes, dendritic cells, and natural killer cells. Immunobiology application of the elispot assay to the characterization of cd81 responses to epstein-barr virus antigens jie yang, victor m lemas, ian w flinn, chris krone, and richard fambinder. Elispot assays are used in a range of applications to monitor immunological response patterns, and their utility for the identification of antigenic peptides has proven useful for the development of immunotherapeutics and vaccine strategies. Analysis of immune cells is the enzyme-linked immunospot (elispot) assay covance uses the elispot technique in applications such as evaluation of vaccine efficacy and immunogenicity of biological.
Elispot assay principle elispot assays employ the sandwich enzyme-linked immunosorbent assay (elisa) technique either a monoclonal or polyclonal antibody specific for the chosen analyte is pre-coated onto a pvdf (polyvinylidene difluoride)-backed microplate.